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نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Effect of various methods of platelet-rich plasma gel preparation on transforming growth factor-p1 release
Effect of various methods of platelet-rich plasma gel preparation on transforming growth factor-p1 release
 
لغة الوثيقة : الانجليزية 
المستخلص : The aim of periodontal therapy is to return the periodontal tissues to a healthy state, to regenerate lost attachment apparatus, and to achieve a gain in clinical attachment level [1]. Periodontal treatment, oral implant surgery, and maxillofacial reconstruction are highly dependent on successful bone regeneration. Bone regenerative techniques, including graft materials, some proteins (as growth factors), and barrier membranes, are often used to improve bone quality or bone quantity before or during these treatments [2,3]. The mechanisms and pathways that govern wound healing and tissue regeneration have been studied. The cellular and molecular events resulting after a traumatic injury are mostly shared by the different tissues of the body and include early and late inflammation phases, proliferation and migration of cells, angiogenesis, granulation tissue formation, and finally matrix formation and remodeling [4,5]. It is assumed that all the phases of tissue repair process are mediated and controlled by a wide range of growth factors (GFs) and cytokines that modulate cell function through direct physical interactions with the extracellular domain of transmembrane receptors. The latter transduce secondary signals, thereby controlling diverse aspects of subcellular biology. Although the role of all the GFs involved in tissue regeneration is only partially explained, the potential benefits of many of them have been shown. For example, platelet-derived growth factor is a powerful mitogen for connective tissue cells [6], transforming growth factor-p (TGF-P) not only stimulates thrombin and calcium were used in either concentration in comparison with other modes of PRP preparation. In conclusion, the highest amount of TGF·~1 released from PRP was obtained at 4-day and 7-day intervals by using thrombin and calcium chloride. Egypt J Oral Maxillofac Surg 2:17-21 @ 2011 The Egyptian Association of Oral and Maxillofacial Surgeons Keywords: animal studies. growth factors. platelet rich, transforming growth factor Department of Oral Medicine, Periodontology, Oral Diagnosis and Radiology, Faculty of Dentistry, Ain Shams University, Egypt Correspondence to Waleed Mohamed Abbas, Assistant Lecturer of Oral Medicine, Periodontology, Oral Diagnosis, Faculty of Dentistry, Ain Shams University, 65, 5th region, EI Tagamoa EI Khames, New Cairo, Helwan, Egypt Tel: +0101418154; fax: +23130551; e·mail: Dentolido@hotmail.com osteoprogenitor cells to proliferate but also blocks in later stages of cell differentiation and mineralization [7], insulin-like growth factor-l might promote the late-stage differentiation and the activity of osteoblasts, and vascular endothelial growth factor induces endothelial cell proliferation and migration, thus initiating the angiogenic response [8]. Some of these GFs are released by platelets that undergo active degranulation [9,10]. Factors released from the platelets include platelet-derived growth factor, TGF-p, platelet-derived epidermal growth factor, platelet-derived angiogenesis factor, insulin-like growth factor, and platelet factor 4 [11]. Platelet-rich plasma (PRP) gel obtained from autologous blood is used to deliver growth factors in high concentrations to the. site of the bone defect or a region requiring augmentation [11]. There are differences in the last step of PRP preparation, which includes the addition of an agent to start gelation and the activation of platelets resulting in the release of a cascade of growth factors from the platelet CI. granules. Some investigators suggested different agents, such as bovine thrombin or fibrin adhesive or sodium alginat~ [12-17]. Although promising results have been obtained with the use of PRP in clinical applications, the optimal calcium and thrombin concentrations for PRP use are still unknown. A study conducted by Lacoste et al. [18] shows that calcium and thrombin induce immediate GF release . from PRP in a dose-dependent manner and suggests that 
ردمد : 2090-097x 
اسم الدورية : Egyptian Journal of oral 
المجلد : 2 
العدد : 1 
سنة النشر : 1411 هـ
2011 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Sunday, March 17, 2013 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
هالة الجابرJaber, Hala باحث مشاركدكتوراه 

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